WebApr 10, 2024 · CTCF expression was upregulated with effector T cell differentiation, with greater induction observed in the Klrg1 hi population by scRNA-seq at day 7 (Figure 2 A). 37 To compare CTCF binding among CD8 + T cell subsets, we performed CTCF chromatin immunoprecipitation sequencing (ChIP-seq) and Cut&Run on sort purified naive, TE, and … WebOct 29, 2024 · Pham et al. uncover mechanisms by which Batf restructures the chromatin landscape during CD4+ effector T cell differentiation. Batf controls Ctcf recruitment to lineage-specifying gene loci in an Ets1-dependent manner to promote chromatin looping and lineage-specific gene transcription, thereby identifying a heretofore unknown …
CTCF–CTCF loops and intra-TAD interactions show differential …
WebThe Myc-associated zinc finger protein (MAZ) was identified and characterized as a regulatory protein associated with MYC gene expression (), and independently as a regulatory factor called Pur-1 that can activate an insulin promoter in HeLa cells ().It was also identified as a serum amyloid A-activating factor and named SAF-1 ().Previous … WebFeb 5, 2024 · To understand CTCF's direct role in global transcriptional regulation, we integrated the miniAID-mClover3 cassette to the endogenous CTCF locus in a human pediatric B-ALL cell line, SEM, and an ... how many miles can you go between oil changes
Tcf1–CTCF cooperativity shapes genomic architecture to promote …
WebThe T-cell receptor (TCR)-alpha (TCRA; see 186880) ... To elucidate the role of CTCF in cell-state transitions and cell proliferation, Stik et al. (2024) studied the effect of CTCF depletion during the conversion of human leukemic B cells into macrophages with minimal cell division. CTCF depletion disrupted TAD organization but not cell ... WebMay 30, 2016 · The intensity of TH expression (corrected total cell fluorescence, CTCF) in neuritic network (by excluding the cell bodies), was quantified in 10 images in triplicates of infected and control ... how many miles can you drive in 50 minutes