Determing c-myb protein level can isolate
WebAug 31, 2024 · (C) Carotid VSMCs isolated from mice homozygous for a hypomorphic c-myb allele (c-myb h/h) have no differences in c-myb expression, but their known reductions in c-Myb activity were associated with reduced expression levels of miR-143 (p<0.01**) and miR-145 (p<0.001***) versus wt. qPCR analysis was performed with n = 3 biological … WebApr 7, 2024 · Watermelon (Citrullus lanatus) is an economically important cucurbit crop. Its pulp is rich in antioxidant carotenoids, which confer a variety of flesh colors. ClPsy1 (Phytoene Synthase) is the rate-limiting enzyme for carotenoid synthesis; however, the promoter activity of ClPsy1 is still unknown. In the present study, promoter sequences …
Determing c-myb protein level can isolate
Did you know?
WebDetermining c-Myb protein levels can isolate functional hematopoietic stem cell subtypes. Sakamoto H; Takeda N; Arai F; et al. See more; Stem Cells (2015) 33(2) 479-490. DOI: 10.1002/stem.1855. 7 Citations. Citations of this article. 30 Readers. Mendeley users who have this article in their library. WebAug 1, 2008 · TLDR. Analysis of c-myb gene transcriptional regulation using two cell lines modeling normal hematopoietic progenitor cells and transformed myelomonocytic blasts …
WebJul 1, 2006 · Myb kd is a floxed-neo cassette inserted into intron 6 of the c-Myb gene. 37 This modification leads to a reduction of c-Myb protein expression to approximately 5% to 10% of the wild-type level. Hematopoietic precursors from 5-FU-treated bone marrow harvested from 8- to 10-week-old mice of the indicated phenotype were transduced with … WebOct 3, 2011 · The Myb oncogene family was discovered because of the retroviral transduction of the chicken cellular myb protooncogene that generated the avian myeloblastosis virus (1, 2).Both the cellular Myb and viral Myb proteins are present within the nucleus of the cell, bind to specific DNA sequences, and can regulate gene …
WebOct 14, 2015 · For instance, hematopoietic stem cells differing in c-Myb protein levels more than 2-fold exhibit no more than 1.3-fold difference in c-myb mRNA levels 35. ... WebFeb 1, 2015 · The transcription factor c-Myb was originally identified as a transforming oncoprotein encoded by two avian leukemia viruses. Subsequently, through the generation of mouse models that affect its expression, c-Myb has been shown to be a key regulator of hematopoiesis, including having critical roles in hematopoietic stem cells (HSCs).
WebOct 14, 2015 · trol levels (Fig. 2B), while c-Myb protein levels were almos t extinguished (Fig. 2C). ese results indicate that miR-150 pr eferentially targets c-M yb translation. Low levels of miR -150 in RMS ...
WebAug 1, 1991 · Various alterations of c-Myb itself may determine the lineage of differentiating hematopoietic cells, and the presence of either the normal amino or carboxyl terminus of c -Myb can suppress transformation when fused to v- myb. The protein product of the v-myb oncogene of avian myeloblastosis virus, v-Myb, differs from its normal cellular … photo of macbook air screenWebDetermining c-Myb protein levels can isolate functional hematopoietic stem cell subtypes. Hiroshi Sakamoto, Naoki Takeda, Fumio Arai, Kentaro Hosokawa, Paloma … how does nato remove a memberWebIt is demonstrated that subtypes of HSCs can be isolated based upon their respective c‐Myb protein expression levels, and how essential it is to include protein studies when … how does native american dna show upWebUsing this reporter line we demonstrate that subtypes of HSCs can be isolated based upon their respective c-Myb protein expression levels. HSCs expressing low levels of c-Myb … photo of magicianWebThe 48-kDa v-Myb protein is truncated relative to the normal 75-kDa c-Myb protein at both its amino and carboxyl termini (Figs. 1 and 2) (Gerondakis and Bishop, 1986; Rosson … how does nationsbenefits workphoto of male prostateWebMay 15, 2024 · The c-Myb transcription factor is required for adult hematopoiesis, yet little is known about c-Myb function during lineage-specific differentiation due to the embryonic … how does nationwide smart miles work