Phospho eif2 alpha antibody

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August undefined, 2024

WebEIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; … WebNov 26, 2024 · Phospho-eIF2α has increased affinity to the eIF2β subunit and prevents the regeneration of guanosine triphosphate (GTP) in the ternary complex eIF2-GTP-Met tRNAi, thus halting the initiation of ...

eIF2 alpha (Phospho-Ser51) Rabbit mAb - SAB Signalway Antibody

WebJun 8, 2024 · The phosphorylation of eIF2α occurs on serine 51 in response to the activation of one out of four eIF2α kinases 4, 5, 6. EIF2AK1, commonly known as heme-regulated … WebBinding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). danish chocolate slices

Human Phospho-eIF2 alpha (S52) Antibody MAB39971: …

WebEIF2a (Eukaryotic translation initiation factor 2A) is a heterotrimer composed of three subunits (alpha, beta, and gamma). This translation initiation factor drives binding of … WebIHC-P analysis of human breast carcinoma tissue using GTX50300 eIF2 alpha (phospho Ser51) antibody. GTX50300 WB Image WB analysis of K562 cells untreated or treated with IFN-α (100ng/ml, 20min) lysates using GTX50300 eIF2 alpha (phospho Ser51) antibody (Lane 3 and 4) and eIF2α antibody (Lane 1 and 2). ... WebWestern blot - Phospho-eIF2α-S51 Rabbit pAb (AP0342) Western blot analysis of extracts of various cell lines, using Phospho-eIF2α-S51 antibody (AP0342) at 1:1000 dilution.HeLa and C6 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014 ... danish chocolate licorice

(9721) Phospho-eIF2α (Ser51) Antibody - CiteAb

Anti-eIF2 alpha (phospho Ser51) antibody (GTX50300) GeneTex

WebEIF2a (Eukaryotic translation initiation factor 2A) is a heterotrimer composed of three subunits (alpha, beta, and gamma). This translation initiation factor drives binding of initiator methionyl-tRNA to the 40S ribosome in an AUG-dependent manner to form the 43S pre-initiation complex. WebSpecificity. This antibody reacts to human eIF2 alpha/EIF2S1 (eukaryotic translation initiation factor 2 subunit alpha) only when phosphorylated at Ser51. There is no cross-reactivity to eIF2 alpha/EIF2S1 without phosphorylation at Ser51. This antibody may also react to mouse or rat Phospho-eIF2 alpha/EIF2S1 (Ser51). Isotype. birthday cake gourmetWebPolyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser51 of human eIF2alpha. Antibodies are purified by protein A and peptide affinity chromatography. Validations None provided Applications Western Blotting (WB) Reactivity Chlorocebus sabaeus (Green monkey) birthday cake gluten free

"WebAnti-EIF2 α Antibody detects endogenous levels of total EIF2 α protein. Immunogen The antiserum was produced against synthesized peptide derived from human eIF2 alpha. Immunogen Range: 21-70 Features and Benefits Evaluate … " - Phospho eif2 alpha antibody

Phospho eif2 alpha antibody

eIF2 alpha/EIF2S1 [p Ser51] Antibody (RM298) - Novus Biologicals

WebWestern Blot. Detection of Human Phospho-eIF2 alpha (S52) by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and HEK293T human embryonic kidney cell line untreated (-) or treated (+) with 100nM Calyculin A (Catalog # 1336) and 20 ng/mL Recombinant Human TNF-a (Catalog # 210-TA) for 10 minutes or 20 … WebEIF2AK1, which is activated by heme deficiency and other stimuli, is a major kinase that phosphorylates EIF2-alpha. Phosphorylation of the alpha subunit of EIF2 downregulates protein synthesis in response to various stress conditions. EIF2AK1 has some isoforms with 630aa, 577aa and 422aa. Protocols Publications All (7) KD/KO (1) WB (7) IP (2)

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WebJul 16, 2024 · eIF2α Antibody (D-3) is an IgG 1 κ mouse monoclonal eIF2 alpha antibody (also designated EIF2A antibody, eIF2 alpha antibody, or EIF2S1 antibody) suitable for the … WebThis clone failed to immunoprecipitate EGFR Phospho (Tyr845) from whole cell extracts prepared from EGF-stimulated A-431 cells. This recombinant antibody clone is derived from clone A18028A. This clone is predicted to recognize the region corresponding to EGFR Phospho (Tyr845) in both rat (Tyr846) and mouse (Tyr847) orthologs due to complete ...

WebProduct Name eIF2 alpha (Phospho-Ser51) Rabbit mAb; Host Species Rabbit; Clonality Monoclonal; Isotype Rabbit IgG; Purification Affinity-chromatography; Applications WB … WebMay 11, 2024 · Human Phospho-eIF2 alpha (S52) Antibody Summary. Detects human Phospho-eIF2 alpha (S52) in ELISAs and Western blots. Lyophilized from a 0.2 μm …

WebPhospho-eIF2α (S51) Polyclonal Antibody detects endogenous levels of eIF2α protein only when phosphorylated at S51. Formulation： Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. Source： Polyclonal, Rabbit,IgG Dilution： IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000.

WebPhospho-eIF2alpha (Ser51) Antibody detects endogenous eIF2alpha only when phosphorylated at Ser51. The antibody does not recognize …

WebMay 13, 2024 · For eIF2 phosphorylation, purified PKR and eIF2 (0.08 µg PKR per 10 µg of eIF2) were mixed at room temperature in eIF2 storage buffer supplemented with 0.5 mM ATP, 10 mM MgCl 2 and 5 mM NaF, and ... birthday cake gifts by postWebProduct name Anti-elF2 alpha (phospho S52) antibody - N-terminal Description Rabbit polyclonal to elF2 alpha (phospho S52) - N-terminal Host species Rabbit Tested … birthday cake g-rated stamphttp://immunoway.com/Home/22/YP0093 danish christmas customsWebAnti-EIF2S1 (phospho S51) antibody [E90] (ab32157) only detects eIF-2a phosphorylated on Serine 51. Tested applications Suitable for: WB, IHC-P, Dot blot more details Unsuitable for: Flow Cyt,ICC/IF or IP Species … danish chocolate chip cookiesWebPhospho-EIF2S1 (Ser51) antibody for WB, ELISA and reacts with Human, mouse, rat. ... eIF 2 alpha, EIF 2A, EIF 2alpha, EIF2, EIF2A, EIF2S1, EIF2S1 (Ser51), EIF2S1 S51, EIF2S1 Ser51, Phospho-EIF2S1 (Ser51), S51, Ser51 ... EIF2S1 is one subunit of the translation initiation factor EIF2, which catalyzes the first regulated step of protein synthesis ... danish chocolate milkWebThe antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns. Storage: … danish christmas candlesWebEIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; … birthday cake graphic free