Thp1 sting haq
WebMar 1, 2024 · N. THP1 cells were stimulated with dsDNA (4 μg/ml) or 2′3′ cGAMP (4 μg/ml). The cells were fixed 8 h later and stained with antibodies against p62, K63-linked ubiquitin, and STING. Scale bar: 5 μm. O. THP1 cells were stimulated with dsDNA (4 μg/ml) for 8 h and stained with antibodies against STING, p62, and beclin-1. Scale bar: 5 μm.
Thp1 sting haq
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Web此外，STING在保护个体发生中的作用已在包括胶质瘤(Ohkuri T等，"Protectiverole of STING against gliomagenesis:Rational use of STING agonist in anti-glioma immunotherapy"Oncoimmunology.2015；4:e999523)和结肠癌(Zhu Q等，"Cuttingedge:STING mediates protection against colorectal tumorigenesis by … Webwhich contained the R232 version of STING was knocked in to THP1 cells in which the HAQ version was ablated, were procured from Invivogen, CA, USA. Stable cell line generation a) …
WebSTING knockout dual reporter monocytes. THP1-Dual™ KO-STING cells were generated from THP1-Dual™ cells by stable knockout of the STING gene. STING (stimulator of interferon genes), alternatively known as … WebAug 21, 2024 · Pharmacological activation of the STING (stimulator of interferon genes)–controlled innate immune pathway is a promising therapeutic strategy for cancer. …
WebDec 5, 2024 · Of note, THP1 cells have the STING HAQ allele found in 20% of population whereas R232 is the STING “WT” allele found in ~60% of population . Modified vaccinia Ankara (MVA) and the BHK-21 cells used to propagate MVA were obtained from ATCC (VR-1508 and CCL-10, respectively). WebThey were generated from THP1-Dual ™ KO-STING cells, which derive from the human THP-1 monocytes, by stable biallelic knockout of the endogenous HAQ hSTING gene and …
WebFurthermore, the variant hSTING haplotype (HAQ) found in the THP1 monocytic cell line has low intrinsic activity but responds to bacterial and metazoan CDNs. Very recently, a splice variant of hSTING, named MRP, has been identified in HeLa and 293T cells that acts as a dominant negative regulator of STING-mediated IFN production10.
WebMar 29, 2024 · The HAQ/HAQ TMEM173 genotype may contribute to the slower disease progression characteristic of LTNPs. This non-canonical activation of STING is mediated by the DNA binding protein IFI16, together with the … craftsman riding lawn mower mulch kitWebSep 30, 2024 · f, THP1 STING R232 or THP1 STING KO cells were incubated with increasing concentrations of cGAMP with or without 250 μM Mn 2+ for 6 h, followed by … divorce lawyers in ocean springs msWebWe constructed plasmid vectors that encode STING-WT, STING-HAQ, or STING-R232H (the third most common STING variant). We then transfected these along with a IFN … divorce lawyers in oneonta nyWebSR-717 lithium is a non-nucleotide STING agonist that demonstrates broad interspecies and interallelic specificity with EC50 of 2.1 μM and 2.2 μM in ISG-THP1 (WT) and ISG-THP1 (cGAS KO) cell lines, respectively. SR-717 lithium also induces the expression of clinically relevant targets, including programmed cell death 1 ligand 1 (PD-L1), in a STING … craftsman riding lawn mower newWebJun 20, 2024 · DNA damage response inhibition does not induce a type I interferon response in THP1 or Jurkat cancer cells. Proliferation of acute monocytic leukaemia THP1 and acute T cell leukaemia Jurkat cells (Supplementary Table 1) was robustly inhibited by the potent Chk1 inhibitor V158411 [].THP1 cells have been routinely used to study the cGAS / STING … divorce lawyers in onslow county ncWebJul 1, 2024 · In recent years, STING has become an attractive therapeutic target for cancer immune therapy and hydrolysis resistant CDNs have been developed as a new class of cancer therapeutics. ... RT-PCR and Western Blotting were performed to evaluate the effect of SR-8314 in 2′3′-cGAMP primed THP1 dual reporter cells (Invivogen). divorce lawyers in ontarioWebJul 1, 2024 · IFNB1 production in THP1 monocyte was induced when THP-1 cells were incubated with the conditioned medium from MDA-MB-231 cells. In this IFNB1 assay, ZXP-8202 had an EC50 of 10nM. To confirm the ZXP-8202 potency in this culture system reflecting cellular ENPP1 activity, MDA-MB-231 was also treated with 2'3' … divorce lawyers in orange tx