Top/fopflash reporter assay

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August undefined, 2024

Web10. jan 2024 · TOP/FOP-Flash reporter assay showed that CDX2 knockdown or CDX2 overexpression significantly increased or decreased Wnt signaling activity. Western blot assay showed that downstream targets... Web86 Upstate Biotechnology Inc topflash reporter Topflash Reporter, supplied by Upstate Biotechnology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 …

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WebLuciferase reporter assays were performed by transfecting TOP/FOP Flash (A), Hes1 (B) or Hey1 (C) luciferase reporter in the cells treated with or without rapamycin (Rapa, 200nM, Sigma), an ... WebJournal: PLoS ONE Article Title: The natural compound Jatrophone interferes with Wnt/β-catenin signaling and inhibits proliferation and EMT in human triple-negative breast cancer doi: 10.1371/journal.pone.0189864 Article Snippet: The activity of β-catenin dependent Wnt signaling was measured by the TOPFLASH reporter using the Dual Luciferase Reporter … reid whiting

Topflash Reporter Assay Bioz Ratings For Life-Science Research

WebThe Luciferase Reporter 1000 Assay System (Cat.#. E4550) was designed to meet the needs of users who perform a large number of assays, particularly in 96-well plates. The system contains sufficient reagents to perform 1,000 luciferase assays (100µl per assay). For users working with transformed cells, a cell lysis buffer will be needed for ... Web一般而言，topflash/fopflash是比较常用的检测β-catenin的系统，原理和所有的RLU一样，都是利用转录因子可以和特异性的靶位点结合进而启动下游的虫荧光素酶基因的表达。如 … Web1169.00元. TOPFlash(报告基因质粒)是用于检测Wnt信号通路中β-catenin介导的TCF/LEF转录活性水平的报告基因质粒。. TOPFlash是以碧云天的pGL6-TA为模板，在其多克隆位点插 … reid wilson anxiety

Topflash Reporter Assay Bioz Ratings For Life-Science Research

Web王志国，饶艳伟(1.赤峰市松山医院骨科，内蒙古赤峰 024005；2.吉林省人民医院重症医学科，吉林长春 130021)骨肉瘤是一种常 Web10. jan 2024 · TOP/FOP-Flash reporter assay showed that CDX2 knockdown or CDX2 overexpression significantly increased or decreased Wnt signaling activity. Western blot assay showed that downstream targets of Wnt signaling, including β-catenin, cyclin D1 and c-myc, were up-regulated or down-regulated in CDX2-knockdown or CDX2-overexpressing … procreate download for ipadWeb6. apr 2024 · The DNA was a mix of 2.25 μg TOP/FOP-Flash reporter and 0.25 μg pRL-TK . The Dual-Luciferase Reporter Assay system (Promega, E1910) was used for collection and luminescence measurement, according to manufacturer's instructions. In short, on day 3 or 4 of differentiation the cells were washed with PBS and 500 μl Passive Lysis Buffer … procreate download for pc

"WebA, B, C. Western blot analysis and TOP/FOP Flash reporter assays confirmed that TP73-AS1/miR-27b-3p/TMED5 axis affected wnt/β-catenin pathway. D. Representative images of the tumor lumps of each ... " - Top/fopflash reporter assay

Top/fopflash reporter assay

Luciferase Reporter Gene Detection Kit Sigma-Aldrich

Web29. jún 2024 · TOP/FOP-Flash luciferase reporter assay and western blot were used to analysis the activation of Wnt/β-catenin signaling pathway. Si-CASC11-transfected HeLa cells were subcutaneously inoculated into male athymic (nude) mice to investigate the effect of CASC11 on the tumor formation. WebTOP/FOP-FLASH assay in HEK293T cells? Has anyone tried to study Wnt signaling in HEK293T cells? I have been trying to measure the Wnt activity using TOP/FOP-FLASH …

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WebThis is a CONTROL luciferase reporter with the TCF/LEF sites of Super8XTOPflash (construct M50, Addgene Plasmid #12456) being mutated. There are 6 mutated TCF/LEF … WebIn our lab, we usually transfect 50ng of DNA per well of a 96 well plate (in which we plate between 10-30K cells, depending cell line). From that 50ng DNA, 15ng are TOPFlash …

WebTOPflash (TCF Reporter Plasmid) Transfection grade T-cell factor reporter plasmid containing 2 sets (with the second set in the reverse orientation) of 3 copies of the TCF binding site (wild type) upstream of the Thymidine Kinase minimal promoter and Luciferase open reading frame. - Find MSDS or SDS, a COA, data sheets and more information. WebThis is a luciferase reporter of beta-catenin-mediated transcriptional activation. In HEK cells, maximal activation of this reporter is ~100-fold (activation by Wnt) up to ~1,000-fold (activation by phosphorylation mutants of beta-catenin). The appropriate control plasmid is clone M51, Super8XFOPflash, which has mutant TCF/LEF binding sites.

Web6. apr 2024 · The TOP/FOP-FLASH assay showed that the canonical Wnt activities were reduced in HONE1 and NPC43 cells, when THY1 was over expressed (Figure 2A). ... Luciferase reporter HONE1 cells were injected into nude mice via tail vein, followed by treatment with 10 mg/kg of KX2-391 or solvent control at a frequency of twice per week … Web背景技术微小RNA（miRNA）在肿瘤的发生和发展中起着至关重要的作用。以前，我们表明miR-504被下调并抑制胶质母细胞瘤（GBM）中的肿瘤增殖。然而，miR-504在GBM间充质（ME）过渡中的调控及其相关机制仍不清楚。方法转录组和临床数据来自癌症基因组图谱（TCGA）数据库。

WebThe protocol I follow is as follows: Day 1 : See 0.2e6 HEK293 Top Flash cells (stable cell lines with Renilla/Luc reporters) in 250uL DMEM-10% FBS, on a 24-well plate. Day 2 : Change media with 0,...

Web1169.00元. TOPFlash(报告基因质粒)是用于检测Wnt信号通路中β-catenin介导的TCF/LEF转录活性水平的报告基因质粒。. TOPFlash是以碧云天的pGL6-TA为模板，在其多克隆位点插 … procreate download gratisWebTo verify our hypothesis, we first used TOP/FOPFlash reporter assays to detect the effect of miR-504 on Wnt-β-catenin signaling pathway activity, and found that it was significantly … reid winthrop attorneyWeb26. dec 2024 · topflash is a tcf reporter plasmid containing two sets of three copies of wild-type tcf binding sites driven by the thymidine kinase minimal prompter and upstream of a … procreate download free for androidWebTop Fop Flash Assay, supplied by Upstate Biotechnology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more ... Article Snippet: The TOP/FOP flash reporter plasmids containing wild-type (CCTTTGATC; TOP flash) or mutated (CCTTTGGCC; ... procreate download free windowsWeb12. apr 2024 · TOP/FOP flash luciferase reporter assay was carried out to detect the activity of Wnt pathway. Besides, qPCR, RNA pulldown and luciferase reporter assays were conducted to probe into the target gene of miR-489-3p. Immunoprecipitation-western blot (IP-western blot) analysis was implemented to assess the effect of USP48 on the … reid withrow obituaryWebThe TOP-flash assay ( Molenaar 1996) is widely used and variants of TOP-Flash (SuperTop) are available from various sources including lentivirus-based reporters from the Nusse … reid winery napaWeb86. Millipore luciferase top flash. Luciferase Top Flash, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, … reid winchester indiana